Gibson SOLA Platform

The Gibson SOLA process enables DNA synthesis on demand. Kits are available off the shelf and can be stored in your facility to enable on demand synthesis.

The Gibson SOLA process enables DNA synthesis in less than a day from digital sequence to dsDNA.

The Gibson SOLA Platform is available in 3 different configurations based on your needs. Please contact Telesis Bio [sales@telesisbio.com] to request a free consultation.

DNA synthesis costs are determined by scale, throughput, and desired capabilities. Please contact Telesis Bio to request a free consultation.

Depending on the configuration of the implementation, the process requires a Echo 525 Acoustic Liquid Handler from Beckman Coulter Life Sciences, a validated standard liquid handler such as the Biomek i7 automated liquid handler from Beckman Coulter Life Sciences. Final gene assembly can also leverage the Telesis Bio BioXp 3250. For more specific equipment requirements, please contact Telesis Bio to request a free consultation.

The Gibson SOLA Platform uses reagents and software that are supplied exclusively by Telesis Bio. The platform is designed to be compatible with most industry standard liquid handlers depending on throughput needs.

- The final assembly step is compatible with the BioXp 3250. For automation of the full process additional hardware is required. Please contact Telesis Bio to request a free consultation.

Gibson SOLA results in synthetic dsDNA constructs with error rates <1:70,000. In addition, the percentage of full-length product in the final pool of DNA will exceed 80% full-length and will often be >95% full-length.

Current specifications enable synthesis of 30% - 60% overall GC with local variation as high as 85%. However, due to the unique block-based assembly process used by Gibson SOLA to synthesize dsDNA, there are multiple approaches that can be used to address GC content outside of these ranges. Please contact Telesis Bio to request a free consultation.

Currently Gibson SOLA supports lengths from 0.3kb to 4kb for de novo synthesis. In addition, all sequences will be automatically reviewed for overly complex regions. These regions will be flagged with an explanation of what was identified. Local optimization tools are provided to address complex regions.

Gibson SOLA is a natively modular system which allows the incorporation of external components. In addition, depending on your needs, the Gibson SOLA software can be incorporated into onsite LIMS to enable further capabilities. Please contact Telesis Bio to request more information.

The Gibson SOLA reagents are split into two modules: the Gibson SOLA DNA Synthesis kit Module 1 and the Gibson SOLA DNA Synthesis kit Module 2. Module 1 contains the stock building blocks and supporting enzymes that enable de novo synthesis of dsDNA. Module 2 contains the reagents necessary for the assembly of the synthesized dsDNA into the desired final construct.

Module 1 and Module 2 can be ordered individually and stored at your facility as needed. Individual components within the modules cannot be ordered independently.

All reagents in the Gibson SOLA Modules 1 & 2 are stock reagents that require no customization. These kits are shipped on dry ice and stored at -80°C.

The Gibson SOLA platform uses a custom design tool that evaluates individual sequences for buildability using the Gibson SOLA method. This tool will highlight any potential issues with the submitted sequence. In addition, we offer optimization tools to address those specific regions that have been identified. If you require further design help, we have a team of application scientists who can provide some additional design insights. Please contact Telesis Bio to request more information.

Telesis Bio offers multiple solutions for sequence analysis and storage depending on your needs. Our standard offering uses a cloud-based portal for sequence submission and analysis. Sequences are stored in a secure, encrypted database only accessible by the account holder. We also offer an onsite software solution that provides unmatched confidentiality and control over sequence information. Please contact Telesis Bio to request a free consultation.