Gibson Assembly® kits
The world’s most trusted method to build seamless high-fidelity DNA
Simple, robust protocols
Seamlessly assemble large, complex constructs
High-fidelity, high-efficiency assemblies
Gibson Assembly® Ultra kits
Contains all the reagents necessary to simultaneously assemble as many as 10 DNA fragments into a vector to produce a final product that is several hundred kilobase pairs in length
Gibson Assembly® RapidAMP™ kits
Contains all the reagents necessary to simultaneously assemble and clone DNA using Gibson Assembly, and then amplify the resultant product up to ~10 µg DNA
Gibson Assembly® HiFi
Product specifications
Maximum # of fragments assembled: 5
Cloning efficiency: 90% full-length clones
Insert size range: 500–32,000 bp
Total construct size range: 100,000 bp
Reaction time: 60 minutes
Gibson Assembly® Ultra
Product specifications
Maximum # of fragments assembled: 10
Cloning efficiency: 95% full-length clones
Insert size range: 100–100,000 bp
Total construct size range: 1,000,000 bp
Reaction time: 80 minutes
Gibson Assembly® RapidAMP™ Ultra kit
Product specifications
Maximum # of fragments assembled: 4
Cloning efficiency: 95% full-length clones
Insert size range: 300–1,800 bp
Total construct size range: 10,000 bp
Reaction time: 80 minutes + 4 hours
Optimize your workflow with the BioXp® system
Revolutionizing synthetic biology
Gibson Assembly® reagents are available in a benchtop reagent kit or in automated format, compatible with the BioXp® 3200 system and BioXp® 3250 System.
The BioXp® system enables up to 32 constructs to be built, cloned into any vector of interest (up to 4 vectors per run), and amplified to > 10 µg transfection-ready DNA in a single overnight run, completely eliminating the need to transform into E. coli.
