DNA cloning
Handsfree, automated cloning and assembly on your benchtop
BioXp® DNA cloning kits
The BioXp DNA Select and De Novo Cloning Kits include all necessary reagents to generate circular plasmids from existing fragments or from digital sequences into a vector of choice.
Utilizing the power of the BioXp system and application-specific BioXp kits, Telesis Bio provides turnkey automation of critical synthetic biology and molecular cloning workflows.
BioXp de novo DNA cloning kits
Built per user provided digital sequence, these kits contain all of the Gibson Assembly® reagents necessary to synthesize and clone error-corrected, de novo synthetic fragments up to 7 kb in length into made-to-stock or custom vectors in a single run on the BioXp platform.
Product specifications
Product format: Circularized double-stranded DNA clones and linear double-stranded DNA fragments
Vector Options: Telesis Bio-provided or user-provided vector of choice
Output size: 300 to 7,200 bp clone insert
Order throughput: 1 to 96 samples per run (up to 32 samples per run on the BioXp 3250)
Configuration: 8, 16, 24, 32, 48, 72, 96 reactions per run (48, 72, 96 rxn sizes exclusive to the BioXp 9600)
Yield: >200 ng per DNA fragment
System runtime: ~12-21 hrs
Total turnaround time: 3 to 5 business days from order processing to shipped kit (International shipping will vary)
Classification: Research use only
BioXp select DNA cloning kits, Gibson Assembly®
Off-the-shelf Gibson Assembly cloning kits enable automated cloning of user-provided fragments into a user-provided vector backbone in a single run on the BioXp platform. These kits contains all of the reagents necessary to generate circularized double-stranded DNA plasmids from input DNA fragments on demand.
Product specifications
Product format: Circularized double-stranded DNA clones
Input format: User supplied fragments of size 300-6000bp from PCR products, DNA variant libraries, or DNA fragments from Telesis Bio, IDT, or Twist Biosciences (Note: input sizes vary depending on source).
Vector Options: Telesis Bio-provided or user-provided vector of choice
Configuration: 8, 24 reactions per kit
System runtime: 2 hours
Turnaround time: Kits ship within 3 business days of order receipt
Classification: Research use only
BioXp select DNA cloning, Golden Gate Assembly
Off-the-shelf Golden Gate assembly cloning kits enable automated cloning of user-provided fragments into a user-provided vector backbone using two of the most popular Type IIS restriction enzymes, BsaI & BsmbI, in a single run on the BioXp platform. These kits contain the necessary reagents to generate circularized double-stranded DNA plasmids from input DNA fragments on demand.
Product specifications
Product format: Circularized double-stranded DNA clones
Input format: User supplied fragments of size 300-1800bp from PCR products, DNA fragments from Telesis Bio, IDT, or Twist Biosciences. Entry vectors containing pre-cloned inserts are also supported.
Vector Options: Telesis Bio-provided or user-provided vector of choice
Configuration: 8, 24 reactions per kit
System runtime: 2 to 7 hours (depending on kit size)
Turnaround time: Kits ship within 3 business days of order receipt
Classification: Research use only
Applications
Antibody drug discovery
Cell & gene therapy
