Libraries
Synthetic DNA, simplified
BioXp® error-corrected libraries kits
BioXp error-corrected libraries kits utilize proprietary technology that combines de novo DNA synthesis, error correction, and amplification. The result is purified library gene fragments ready for downstream applications such as cloning, screening, or selection.
Leveraging the power of push-button, end-to-end automation of the BioXp system, these error-corrected libraries are assembled in a single instrument run of less than 16 hours. This process is efficient regardless of design complexities, enabling researchers to significantly reduce synthesis bottlenecks during Design-Build-Test cycles of lead optimization.
These kits are available on both the BioXp 3250 and 9600 platforms.
Scanning libraries
BioXp scanning error-corrected libraries offer a wide variety of libraries with varied amino acid sites which can be categorized into simple-scanning (single amino acid/codon substitutions per well) and site-saturation libraries that employ NNN, NNK, and NNS degenerate codons.
Quickly identify AA sites that are sensitive to effect
Product specifications
Product format: Linear dsDNA
Median error rate: 1 in 5,000 bp
Size range: 300-800 bp*
GC content: 30-60%**
Degenerate codons: NNN, NNK, NNS
Max # degenerate codons: 3 contiguous, 6 non-contiguous per sequence
Configuration: 8, 16, 24, 32, 48, 72, or 96 synthesis reactions
Yield: >200 ng per library construct
System runtime: Up to 16.5 hrs
Classification: Research use only
* - Lengths of < 2,000 bp may be ordered, but not guaranteed
** - GC content 20% < X < 70% may be ordered, but not guaranteed
Combinatorial libraries
BioXp combinatorial error-corrected libraries offer a wide variety of libraries with varied amino acid sites which include NNN, NNK, and NNS degenerate codons as well as degenerate bases from the IUPAC code.
Identify the optimal combination for the desired effect
Product specifications
Product format: Linear dsDNA
Median error rate: 1 in 5,000 bp
Size range: 300-800 bp*
GC content: 30-60%**
Degenerate codons/bases: NNN, NNK, NNS + IUPAC nucleotides
Max # degenerate codons: 3 contiguous, 6 non-contiguous per sequence
Configuration: 8, 16, 24, 32, 48, 72, or 96 synthesis reactions
Yield: >200 ng per library construct
System runtime: Up to 16.5 hrs
Classification: Research use only
* - Lengths of < 2,000 bp may be ordered, but not guaranteed
** - GC content 20% < X < 70% may be ordered, but not guaranteed
Applications
Antibody drug discovery
Cell & gene therapy
