DNA amplification
Build and amplify complex DNA constructs quickly
BioXp™ RapidAMP™ cell-free DNA amplification kit
BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes, cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.
Through the power of the BioXp™ system and application-specific BioXp™ kits, Telesis Bio enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.
Cloning without E. coli — get to your results faster
Get to your results faster and in fewer steps with Gibson Assembly® RapidAMP™ isothermal amplification. Assemble and amplify transfection-ready DNA in a simple two-day workflow. Achieve over 10 µg of high-quality, high-fidelity DNA with RapidAMP™ technology, and skip the tedious tasks associated with transformation, cell culture, and E. coli harvest.
A robust amplification workflow
Begin with a linearized vector, plus up to two linear DNA insert fragments — 300 bp to 1,800 bp each — and assemble via Gibson Assembly® technology to generate a circular cloning product.
This representative gel shows clean bands, post-restriction digest and before any DNA purification. After cloning and amplification on the bench, or with the BioXp™ system, the final DNA product can be digested to linear form. It is then ready for downstream applications, negating the need for traditional E. coli transformation and plasmid purification. RapidAMP™ technology has been used successfully for transfection — lipofection and electroporation — and in vitro transcription. The Gibson Assembly® RapidAMP™ workflow produces clean, high-yield DNA material in only a few hours.
BioXp™ RapidAMP cell-free DNA amplification kit
BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.
Product specifications
Product format: Branched double-stranded DNA and linear double-stranded DNA fragments
Vector options: Telesis Bio vector or your own
Output size: 300-3600 bp (insert size)
Price: Contact us
Order throughput: 1 to 24 samples per run
Configuration: 8, 16, and 24 reactions per run
Yield: >9 µg per reaction
System runtime: ~24 hours
Total turnaround time: 3 to 5 business days from order to shipped kit (International shipping will vary)
Classification: Research use only
