mRNA synthesis
Automate and accelerate DNA sequence to mRNA
BioXp® kits for mRNA synthesis
The BioXp system along with custom-built consumable BioXp kits, automates the synthesis of mRNA streamlining the overall workflow and expediting the time to product to just days instead of weeks or months. The available kits for mRNA synthesis enable flexibility in input so researchers can select a 1-step or 2-step workflow for mRNA synthesis.
BioXp De novo mRNA synthesis kit |
BioXp Select mRNA synthesis kit |
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Input |
The BioXp mRNA synthesis kit combined with the BioXp system provides the first push-button, walk-away end-to-end automation solution for the production of synthetic mRNA from DNA sequence. | The BioXp Select mRNA synthesis kit enables the synthesis of mRNA from an input DNA template. |
Workflow |
It automates the entire workflow including the complex and time-consuming process of synthesizing the DNA template for the generation of the final synthetic mRNA product. | When combined with the BioXp DNA cloning kit it provides a rapid, 2-step automated workflow for synthesis of synthetic mRNA. |
Output |
Purified, capped and tailed synthetic mRNA | Purified, capped and tailed synthetic mRNA |
Runtime |
~ 18 hours | ~ 4-6 hours depending on length |
| Download flyer | Download flyer |
BioXp De novo mRNA synthesis kit
Th BioXp De novo mRNA synthesis kit enables the synthesis of purified mRNA upto 1.8kb from digital sequence. This kit contains all of the reagents necessary to produce capped and tailed synthetic mRNA along with the error-corrected template from digital sequence.
Product specifications
Product format: Single-stranded capped and tailed mRNA, and linear double-stranded DNA fragments
Features: 5’ ARCA or CleanCap® AG (3’OMe); poly(A) tail (median 150 As)
Nucleoside modification options:
- When selecting ARCA: None or Pseudouridine
- When selecting CleanCap® AG (3’OMe): None or N1-methyl-pseudouridine
Price: Contact us
Order throughput: 1 to 16 samples per run
Configuration: 8 and 16 reactions per run
Yield: Minimum 5 µg per mRNA for CleanCap® AG (3’OMe) modified mRNA and minimum 1 µg per mRNA for 5’ ARCA modified mRNA
Total turnaround time: 5-7 business days from order to shipped kit ( international shipping will vary)
Classification: Research use only
BioXp Select mRNA synthesis kit
The BioXp Select mRNA synthesis kit enables you to synthesize purified capped and tailed mRNA between 0.4-10kb. The kit contains all the necessary reagents for on-demand synthesis of mRNA using a linear DNA template generated with a plasmid or PCR product for transcription.
Product specifications
Product format: Contains reagents necessary to synthesize at least 50 micrograms of capped and tailed synthetic mRNA using plasmid or PCR purified DNA template.
Features: 5’ CleanCap® AG (3’OMe) capping
Nucleoside modification options:
- Unmodified or N1-methyl-pseudouridine
Price: Contact us
Order throughput: 1 to 48 reactions per run
Configuration: 8, 16, 24 and 48 reactions per run
Yield: Minimum yield 50 µg mRNA per well
Classification: Research use only