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DNA cloning

Handsfree, automated cloning and assembly on your benchtop

BioXp® DNA cloning kits

BioXp DNA Select and De novo cloning kits provide all of the necessary reagents to generate circular plasmids from existing fragments or digital sequence respectively into a vector of choice.

Through the power of the BioXp system and application-specific BioXp kits, Telesis Bio enables turnkey automation of important synthetic biology and molecular cloning workflows.

BioXp De novo cloning kits

De novo

Built per user provided digital sequence these kits contain Gibson Assembly® reagents necessary to synthesize and clone error-corrected, de novo synthetic fragments up to 7 kb in length into made-to-stock or custom vectors in a single run on the BioXp platform.

BioXp Select cloning kits

Select

Off-the-shelf cloning kits enable automated cloning of user-provided fragments into a user-provided vector backbone using Gibson Assembly or Golden Gate assembly methods in a single run on the BioXp platform.

BioXp De novo DNA cloning kits

BioXp DNA cloning kits contains all of the Gibson Assembly® reagents necessary to make error-corrected, de novo synthetic genes of up to 7 kb in length. These can use either the standard made-to-stock vectors or your specific custom vector.

Product specifications

Product format: Circularized double-stranded DNA clones and linear double-stranded DNA fragments
Vector Options: Telesis Bio vectors or your own
Output size: 300 to 7,200 bp clone insert (up to 3,600 bp on the BioXp 9600)
Order throughput: 1 to 96 samples per run (up to 32 samples per run on the BioXp 3250)
Configuration: 8, 16, 24, 32, 48, 72, 96 reactions per run (48, 72, 96 rxn sizes exclusive to the BioXp 9600)
Yield: >200 ng per DNA fragment
System runtime: ~12-21 hrs
Total turnaround time: 3 to 5 business days from order to shipped kit (International shipping will vary)
Classification: Research use only

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BioXp Select DNA cloning kits

BioXp Select DNA cloning kits contain all of the Gibson Assembly® reagents or Golden gate assembly reagents necessary to generate circularized double-stranded DNA plasmids from input DNA fragments on-demand.

Product specifications

Product format: Circularized double-stranded DNA clones
Input format: User supplied fragments of size 125-3000bp (PCR products, DNA fragments from Telesis Bio, IDT, or Twist Biosciences.)
Vector Options: Telesis Bio provided or user provided vector of choice
Order throughput: 1 to 24 reactions per run
Configuration: 8, 24 reactions per run
System runtime: ~3-7 hrs
Turnaround time: Kits ship within ~3 business days of order receipt
Classification: Research use only

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